BioScope Resolve. The Most Advanced AFM for Life Science Research. Andrea Slade, PhD. Senior Life Sciences Applications Scientist. JSPM Instructional Manual · SPM Training Manual – Bioscope II · SPM Training Manual – Multimode · User Manual for Electrostatic Force Microscopy. Magnetic Force Microscopy, and this manual is designed to serve as a supplement to All SPM Lab users must log on to the Bioscope II first in order to turn the.

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Kowalski Read Me First! Users are cautioned NOT to attempt adjustments to the laser, its electronic control circuits, or optical components. If laser malfunction is suspected, immediately return the entire DimensionTM head to Digital Instruments for repair or replacement; the Dimension head is NOT manal user serviceable part.

The use of optical instruments with this product will increase eye hazard.

This is an exit point for light—never stare into the end of the scanner tube while the laser is on. Do not stare into beam. When covers are removed, laser light may be emitted.

Note that the head features a tilt switch to automatically power down the laser whenever the head is tilted out of a vertical position—if the head is plugged in, then rotated from a horizontal to a vertical position, it will emit a laser beam.

Unplugging tipholders from the end of the scanner tube removes a partial barrier to the laser beam; tipholders should always be removed by pulling straight down, away from the operator. This button is not an emergency stop button, rather this button will raise the hood door in emergency situations.

Important to BioScope Users! Unused accessory ports located on optical microscopes feature removable covers; when covers are removed, laser light is emitted. Call Digital Instruments today for additional safety labels and technical information. Leaving the NanoScope controller ON without a computer connection for extended periods may damage the scanner.

Warning statements identify conditions or practices that could result in bodily injury or loss of life. Caution statements identify conditions or practices that could result in damage to the NanoScopeequipment or other property, and in extreme cases, possible personal injury. Attention to these notes is warranted to insure proper instrumentation technique and accurate data. This manual, as well as the software described therein, is furnished under license and may only be used or copied in accordance with the terms of such license.

Users are advised that, except as permitted by such license, no part of this publication or software may be reproduced, stored in a retrieval system, or transmitted, in any form or by any means, electronic mechanical, recording, or otherwise, without the prior written permission of Digital Instruments, Inc. The information in this manual is furnished for informational use only, is subject to change without notice, and should not be construed as a commitment by Digital Instruments, Inc.

Unless otherwise directed by the technical support staff of Digital Instruments, Inc. Units requiring service or upgrading should be shipped directly to Digital Instruments, Inc. Direct all questions regarding servicing, parts replacement and peripheral equipment to the support staff of Digital Instruments, Inc. Service contracts are also available to extend this warranty period. To validate your warranty or service contract, you must pay careful attention to all Cautions and Warnings included in this and other manuals received from Digital Instruments.

Failure to follow these warnings or other misuse of your NanoScope system may invalidate your warranty or service contract. A Lire Avant Usage! Arbeiten mit dem Laser! Es handelt sich dabei nicht um einen Notausschalter.

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Kameras sind von Digital Instruments zu beziehen. Lilian Capel, Commercial Dept. Emile Gillieron 9, Tzavella St. Customers may refer to the document guide below for locating documentation used with its product line. The manual is organized by real-time bioscopr off-line commands, and includes descriptions of the algorithms used to process image data. Although manuals for older, NanoScope II series are no longer updated, all Nanoscope III manuals are periodically updated to accord with the latest software releases.

Bioscope Manual for SWI – TINATOONS

Most of our application notes can be viewed on our web site http: Training and compliance with all safety requirements is essential during installation and operation of the NanoScope SPM. Figure Safety Symbols Key Symbol Definition This symbol identifies conditions or practices that could result in damage to the equipment or other property, and in extreme cases, possible personal injury. Dieses Symbol beschreibt Zustaende oder Handlungen die das Geraet oder andere Gegenstaende beschaedigen koennen und in Extremfaellen zu Verletzungen fuehren koennen.

Dieses Symbol beschreibt Zustaende oder Handlungen die einen elekrischen Schock verursachen koennen. Biiscope symbol identifies a laser hazard.

During and prior to set up of the laser, it is especially important to avoid looking directly at the laser beam or at the laser spot.

BioScope™ Instruction Manual

The laser head should never be plugged into the microscope control electronics unless the head is installed in the Z-stage mount. Der Laser bioscops niemals an die Mikroskopelektronik angeschlossen werden, wenn er nicht in der Halterung der Z-Verschiebeeinheit installiert ist. Ignition of the UV arc lamp plasma may send multikilovolt spikes into the AC power, affecting sensitive digital and control electronics. After the arc lamp is turned off, allow at least 15 minutes for it to cool before relighting the lamp.

Restarting a still-hot lamp can cause arcing and damage to the electronics. Fluids must NOT be spilled on or around the sample stage, electronics boxes, or other components containing electronic parts. Always handle the AFM head with care—it may be damaged if dropped or crashed with force into samples.

Carefully position the objective as high as it will go without contacting the sample, then focus downward until the sample comes into view. Tips and lenses can be damaged when focusing. Withdraw the probe from the top surface before making focus adjustments. Always lower objective lenses by turning the focus knob before rotating the lens turret. Bitte immer das Objektiv mit der Fokussiereinrichtung herunterfahren bevor der Objektivrevolver gedreht wird.

Use extreme caution moving the X-Y stage if the sample holder has a lip on it that extends above the sample surface like a petri dish. The SPM head can be destroyed if the sample stage is moved such that the tip holder crashes sideways into the lip of your sample holder.

Also, check that the position of the lip of the sample holder will not interfere with the movement of the Z-stage. Use caution when working in Force Calibration mode. A la suite de problemes occasionnels, certains utilisateurs ont experimentes des fuites du joint de la cellule liquide.

Il est donc recommande d’eviter les immersions prolongees dans un liquide. Une fois l’experience termine, veuillez enlever la cellule de la solution, la retirer de la sonde de balayage piezo et la ranger apres sechage.

Permanent damage to the scanner tube may occur. Ceci ne doit jamais arriver. DI recommends using Force Plot only with extreme caution. Set this parameter to a small value before engaging to avoid possibly striking the surface and harming the tip.

The Interleave Drive frequency can also be adjusted, which may be useful if acquiring amplitude data in LiftMode; see Chapter Before enabling the Interleave Drive Amplitude, check that its value is not much larger than the main Drive Amplitude value to prevent possible damage to the tip. Do not insert a conducting object e. Chapters 6—13 cover all basic imaging techniques performed by the BioScope.

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Chapter 10 covers Lateral Force Microscopy LFMa technique used to image the surface friction characteristics of materials. Introduction Chapters 12 and 13 are complementary and should be read together. The last two chapters of this manual are service-oriented and will be used only occasionally. Chapter 14 discusses calibration procedures used to ensure that image dimensions are truly represented. Chapter 15 is a troubleshooting guide.

The manual also contains a tab-indexed table of contents to reduce searching times. Side margin icons are used within some chapters to divide information according to image type TappingMode, contact AFM, etc. Readers may search for these icons to locate information on the imagery of interest. Introduction The BioScope is an exciting new tool for the investigation of biological surfaces. The optical microscope allows quick location of regions of interest on a sample which can then be imaged at higher resolution with the scanning probe microscope.

The microscope SPM head assembly may also be detached and operated separately from the inverted microscope for larger samples see Figure 3. The SPM creates high-resolution images of a sample surface by scanning a sharp tip over the sample.

Bioscope Manual for SWI

Images of the sample surface are created by raster-scanning the cantilever and tip across the surface while monitoring variations in the cantilever motion. The results of the cantilever measurements are recorded and can be displayed as false color images or as threedimensional plots.

The BioScope system consists of a rigid metal frame that suspends the Dimension SPM head above the inverted optical microscope. The SPM head may be moved towards the sample or retracted from the sample with an automated and motorized translation stage.

The BioScope is provided with metal sample holders for glass slides and petri dishes. These sample holders mount under the SPM head and can be translated with a manual X-Y stage, located on the right side of the BioScope. Introduction Normally the user positions the sample until a region of interest is located under the Majual tip, then bioscoe SPM is engaged onto the region of interest.

With optional cantilever tips and tipholders, additional capabilities such as magnetic force microscopy MFMelectric force microscopy EFMand force modulation are available. Microscope Head The Dimension SPM head provides accurate imaging of a stationary sample while scanning the integrated detector probe assembly above the sample.

The Dimension head is very effective in imaging large samples too large or heavy to scan by movement of the sample. Patents covering the technology developed in this head are pending.

The beam emitted by the laser diode 1. The integrated scanner head consists of the following subassemblies: Laser Diode Stage— A kinematic tilt stage is used to position the laser beam onto the cantilever. It consists of the laser diode, collimator, focusing lens, baseplate, and the X and Y laser diode positioners.

The X positioner knob bisocope the beam parallel to the major axis of the cantilever substrate. The Y positioner knob moves the beam perpendicular to the major axis of the cantilever substrate.

The use of these positioners is described in Chapter 5.